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Picture of LightCycler<sup>&reg;</sup> 480 High Resolution Melting Master

LightCycler® 480 High Resolution Melting Master

Ready-to-use hot start reaction mix for real-time PCR and high resolution melting curve analysis using the LightCycler® 480, LightCycler® 96 and LightCycler® Nano Instrument.

For life science research only. Not for use in diagnostic procedures.

Product No. Pack Size List Price Your Price Quantity
04909631001
500 x 20 µL reactions $ 440.00

The LightCycler® 480 High Resolution Melting Master is designed for research studies in combination with the LightCycler® 480, LightCycler® 96 and LightCycler® Nano Real-Time PCR System. It is supplied as a ready-to-use 2x-concentrated hot start reaction mix. A separate 25 mM MgCl2 stock solution, supplied with the master, allows users to easily optimize the Mg2+ concentration. The LightCycler® 480 High Resolution Melting Master can be used in conjunction with Uracil DNA Glycosylase for carryover prevention during PCR.

 

  • Achieve consistent, high quality performance with a master mix containing ResoLight, a novel dye that does not inhibit amplification enzymes or interfere with PCR when added during reaction setup.
  • Take advantage of an optimized master mix that is highly stable and robust in handling, ideally suited for use in automated workflows.
  • Save time with a convenient, ready-to-use 2x concentrated hot start master mix. 

Contents

1. Master Mix, 2x concentrated, including ResoLight high-resolution melting dye
2. MgCl2 Stock Solution, 25 mM
3. Water, PCR Grade

The LightCycler® 480 High Resolution Melting Master provides reagents, including a hot start PCR enzyme and ResoLight, a novel saturating DNA dye, for amplification and detection of heteroduplex regions in PCR amplicons (e.g., in mutation scanning or genotyping applications). The master mix is optimized for use with the LightCycler® 480, LightCycler® 96 and LightCycler® Nano System and can also be used on other instruments with suitable optics. Data capture can be performed using the detection channel for SYBR Green I assays. The LightCycler® 480 High Resolution Melting Master is compatible with PCR additives (e.g., DMSO) that enhance amplification of GC-rich sequences.

Contents

1. Master Mix, 2x concentrated, including ResoLight high-resolution melting dye
2. MgCl2 Stock Solution, 25 mM
3. H2O, PCR-grade

Product No: 04909631001

LightCycler® 480 Real-Time PCR System in Human Genetics

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High Resolution Melting: Optimization Strategies

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Microsatellite Analysis of Grapevine Varieties by High-Resolution Amplicon Melting Analysis

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Mutation Scanning Using High Resolution Melting or dHPLC: a Performance Comparison Study

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Rapid and Reliable Methylation Detection in Archival Tissue Samples using High-Resolution Melting Analysis

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Rapid High-Throughput Methylation Analysis Using the LightCycler® 480 System

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Transfering PCRs to HRM-assays on the LightCycler® 480 System- Examples for BRCA1

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Genotyping using digital PCR with the LightCycler® 1536 System to detect the IDH1 mutationin plasma samples

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