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Search results for "reverse transcription" (38)

LightCycler® EvoScript RNA Probes Master

The LightCycler® EvoScript RNA Probes Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.

For general laboratory use.

LightCycler® EvoScript RNA Probes Master

The LightCycler® EvoScript RNA Probes Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.

For general laboratory use.

cDNA Synthesis System

Optimized one-tube procedure for the synthesis of double-stranded cDNA up to 3 kb from total RNA or mRNA.

For life science research only. Not for use in diagnostic procedures.

EvoScript Reverse Transcriptase

Easy-to-use reaction mix for reverse transcription

For general laboratory use.

EvoScript RNA Probes Master

The EvoScript RNA Probes Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.

For general laboratory use.

Publications (4)

Rapid quantitative detection of TE-AML1 fusion transcripts in pediatric acute lymphoblastic leukemia by real-time reverse transcription polymerase chain reaction using fluorescently labeled probes 

Keywords: LightCycler DNA Master Hybridization Probes; LightCycler FastStart DNA Master Hybridization Probes

P. Bolufer, E. Barragan, A. Verdeguer, J. Cervera, J. M. Fernandez, I. Moreno, E. Lerma, C. Esquembre, M. Tasso, V. Fuster, M. Bermudez and M. Sanz

Haematologica, 87, 1, 23 - 32

Sensitive and Specific Quantitative Detection of Rotavirus A by One-Step Real-Time Reverse Transcription-PCR Assay without Antecedent Double-Stranded-RNA Denaturation 

Keywords: MagNA Pure 96; MagNA Pure Compact

S. Mijatovic-Rustempasic, K. I. Tam, T. K. Kerin, J. M. Lewis, R. Gautam, O. Quaye, J. R. Gentsch and M. D. Bowen

J Clin Microbiol, 2014, 52, 8

Use of the MagNA pure LC automated nucleic acid extraction system followed by real-time reverse transcription-PCR for ultrasensitive quantitation of hepatitis C virus RNA 

Keywords: MagNA Pure LC; Probe

L. Cook, K. W. Ng, A. Bagabag, L. Corey and K. R. Jerome

J Clin Microbiol, 42, 9, 4130-6

Validation of Miniaturized One-Step Reverse Transcription qPCR Assays for High-Throughput Screening and Comparison to a Reporter Gene Methodology 

Keywords: Light Cycler® 480, Light Cycler® 1536, RealTime ready Cell Lysis kit, RealTime ready RNA Virus master

C. Bardelle, L. McWilliams, S. Mounfield, M. Wigglesworth, K. Rich

94-101. doi:10.1089/adt.2014.630

Documents (15)

Reverse Transcription Technology

Transcriptor High Fidelity cDNA Synthesis Kit - Accuracy Meets RT-PCR

The kit not only reduces the amount of errors in RT-PCRs, it also allows the reverse transcription of full-length cDNAs with high yield.

(Product Review, PDF, 184 KB)

Transcriptor Universal cDNA Master - Fast cDNA Synthesis for qRT-PCR

Reduce your reverse transcription time to 20 minutes by using the Transcriptor Universal cDNA Master - a convenient, ready-to-use master mix for fast cDNA synthesis in two-step real-time RT-PCR.

(Sell Sheet, PDF, 1999 KB)

Transcriptional Organization of the O Antigen Biosynthesis Cluster in the GC-Rich Bacterium Burkholderia cenocepacia

We investigated the transcriptional organization of this cluster by RT-PCR analysis using the Transcriptor Reverse Transcriptase with primers that allowed us to detect co-transcription between genes.

(Article, PDF, 215 KB)

Improved Performance of the LightCycler FastStart DNA MasterPLUS SYBR Green I Kit in Highly Sensitive Transcript Quantification

Real-time reverse transcription-PCR (RT-PCR) is considered the method of choice for any experiments requiring sensitive, specific, and reproducible quantification of mRNA molecules of interest. It is an essential tool for basic research, molecular medicine, and biotechnology.

(Article, PDF, 289 KB)
Product Information (3)

Roche Life Science | Reverse Transcription

Get accurate representation of RNA pools for your genomic analysis using Roche's comprehensive selection of reverse transcription products.

Roche Life Science | Products

Explore our complete portfolio of trusted products for working with DNA, RNA, cells and proteins.

Roche Life Science | Techniques And Applications

Explore the innovative techniques and applications Roche supports with their broad portfolio of high-quality products.


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