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Picture of Universal ProbeLibrary Probes

Universal ProbeLibrary Probes

Universal ProbeLibrary Probes 81 to 90
Short hydrolysis probes for quantification of gene expression levels by real-time PCR.
For life science research only. Not for use in diagnostic procedures.

Product No. Pack Size List Price Your Price Quantity
04689046001
Probe #81 $ 185.00

Product No. Pack Size List Price Your Price Quantity
04689054001
Probe #82 $ 185.00

Product No. Pack Size List Price Your Price Quantity
04689062001
Probe #83 $ 185.00

Product No. Pack Size List Price Your Price Quantity
04689089001
Probe #84 $ 185.00

Product No. Pack Size List Price Your Price Quantity
04689097001
Probe #85 $ 185.00

Product No. Pack Size List Price Your Price Quantity
04689119001
Probe #86 $ 185.00

Product No. Pack Size List Price Your Price Quantity
04689127001
Probe #87 $ 185.00

Product No. Pack Size List Price Your Price Quantity
04689135001
Probe #88 $ 185.00

Product No. Pack Size List Price Your Price Quantity
04689143001
Probe #89 $ 185.00

Product No. Pack Size List Price Your Price Quantity
04689151001
Probe #90 $ 185.00

Each Universal ProbeLibrary probe is a single hydrolysis probe that recognizes sequences from one or more of the following organisms: humans, chimpanzees and other primates, mice, rats, C. elegans, Drosophila, Arabidopsis.

Each of the Universal ProbeLibrary probes:

  • Significantly reduces assay design time and implementation time.
  • Includes features that make probe-based assays specific, flexible, and convenient.
  • Needs no special hardware or unusual reaction conditions.

Contents

Two vials, each containing 125 µL of one probe solution [10 μM solution of a single probe, 5' labeled with fluorescein (FAM) and 3' labeled with a dark quencher dye].

Use the Universal ProbeLibrary Probes to obtain rapid, flexible quantification of expression levels for virtually any gene from the human, mouse, rat, chimpanzee, C. elegans, Drosophila, or Arabidopsis transcriptomes in the NCBI Reference Sequence Database. Each probe can be used with standard reagents in real-time PCR assays:

  • on the LightCycler® Carousel-Based System (e.g., with the LightCycler® TaqMan® Master), or
  • on other commercially available real-time PCR instruments (e.g., with the FastStart TaqMan® Probe Master).

Note: You can easily design gene-specific quantification assays using these probes and the free web-based ProbeFinder software at the Assay Design Center.

 

Probe chemistry: Universal ProbeLibrary probes use a unique nucleotide chemistry called LNA (Locked Nucleic Acid), which allows very short (8-9 bases) oligonucleotides to be effective hybridization probes in real-time PCR assays. LNA allows the melting temperature of the short probes to be unusually high. Thus, they are fully compatible with commonly used PCR conditions and the hydrolysis-probe detection format. These probes are even sensitive enough to detect single-base mismatches.

Assay design: The free online ProbeFinder software, available at the Universal ProbeLibrary Assay Design Center, designs one or more intron-spanning assays for a target gene, based on specific information (i.e., gene name, accession number, or sequence) that you submit. For each assay it designs, the software will specify a set of specific primers plus a probe from the Universal ProbeLibrary that will give the best results. The combination of primers and probe will provide specific amplification and detection of your target sequence in a standard real-time PCR assay.

Labeling: pre-labeled with a reporter fluorophore (FAM) and a dark quencher dye. 

Instrumentation required: standard real-time PCR instrumentation.

Notes:  In addition to being available individually, the Universal ProbeLibrary probes are available in sets (90 probes/set), each of which covers virtually all the transcripts from a single organism. Together, the sets cover approximately 99% of the human, primate, mouse, rat, C. elegans, Drosophila, and Arabidopsis gene transcripts listed in the NCBI Reference Sequence Database.
For more information, see the listings for these organism-specific sets.
 

Quality

Function test: All Universal ProbeLibrary probes are tested for performance in real-time PCR using primers and control templates from the Universal ProbeLibrary Control Set. Each probe must pass this test.

Purity: Each probe is analyzed by anion-exchange HPLC.

Composition: Each probe is analyzed by MALDI-MS.

Product No: 04689046001
Product No: 04689054001
Product No: 04689062001
Product No: 04689089001
Product No: 04689097001
Product No: 04689119001
Product No: 04689127001
Product No: 04689135001
Product No: 04689143001
Product No: 04689151001

Comparison of qPCR Assays Using SYBR Green I Intercalation or the Universal ProbeLibrary as Detection Format

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Easy and Reliable qRT-PCR Analysis of Total RNA Isolated from Fresh Frozen and FFPE Tissue Samples

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High-Throughput Cardiac Gene Expression Analysis Using UPL and the LightCycler® 1536 Real-Time PCR System

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ProbeFinder Quick Reference Guide

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Universal ProbeLibrary

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Universal ProbeLibrary Set: One Transcriptome - One Kit

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Universal ProbeLibrary System User Statements and Application

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