RealTime ready Human Apoptosis Panel, 96
Panel of qPCR assays for use on the LightCycler® 480 Instrument to perform gene expression profiling of 84 human genes involved in apoptosis.
For life science research only. Not for use in diagnostic procedures.
The panel consists of 84 pretested, ready-to-use qPCR assays, supplied in a LightCycler® 480 Multiwell Plate 96, white. Each assay includes the appropriate primers and a Universal ProbeLibrary probe that contains Locked Nucleic Acid (LNA). The only components that need to be added are sample cDNA and reaction mix (e.g., LightCycler® 480 Probes Master).
The panel also contains seven human reference gene assays (ACTB, β2M, GAPDH, HPRT1, RPL13A, 18S, and YWHAZ), which enable relative quantification of the apoptosis-related genes. In addition the panel provides three positive- and two negative control assays for the reverse transcription (RT) reaction. The RT positive control is used to determine if degradation of your initial RNA sample has occurred and to assess the quality of your RT step. The RT negative control detects residual genomic DNA.
Be confident in your results:
Controls are provided and assays are pretested to ensure optimal performance.
Save time with a fast and efficient workflow:
The convenient pre-plated assays require only the addition of sample cDNA and master mix.
Easily import sample information directly to the LightCycler® 480 Instrument:
Plate configuration details can be accessed online and downloaded to the LightCycler® 480 Software.
Perform relative quantification of target genes:
Seven reference gene assays are included on each plate.
One package containing two LightCycler® 480 Multiwell Plates 96, white and Sealing Foils. Each plate contains assays for 84 different apoptosis-related genes and seven housekeeping genes, as well as five controls.
For the plate layout, please see the attached pdf 'Plate Layout'. To import the sample information to your LightCycler® 480 software 1.5 sample editor, please open the attached file 'Macros Apoptosis 96.zip' to either download the LightCycler® 480 Experiment Macro, appropriate for your LightCycler® 480 System I or II, containing the sample information and a complete run protocol or the txt. file containing the sample information only.
Use the RealTime ready Human Apoptosis Panel, 96 to quantify the expression levels of 84 human apoptosis-related genes. This gene expression profiling panel can be assayed directly on a LightCycler® 480 Instrument.
Additional RealTime ready Focus Panels:
The panel is designed for expression profiling of genes that play a major role in apoptotic processes in human cells. On the plate, assays for the 84 apoptosis-related genes are grouped to reflect the various protein families and pathways involved in apoptosis:
|Caspase family||CARD family|
|Bcl-2 family||TNFR family|
|IAP family||NF-κB signaling pathway|
|p53 signaling||Fas signaling pathway|