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Picture of Rabbit Anti-Human Vimentin Monoclonal Antibody (Clone SP20)

Rabbit Anti-Human Vimentin Monoclonal Antibody (Clone SP20)

For research use only. Not for use in diagnostics.

Product No. Pack Size
05499917001
0.1 mL

Product No. Pack Size
05298784001
1.0 mL



Vimentin is the main intermediate filament protein in mesenchymal cells. This protein is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin stains sarcomas of neural, muscle and fibroblast origin, but carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin.

Figure above:
Human Melanoma stained with anti-Vimentin antibody

Contents

Rabbit monoclonal antibody supplied as tissue culture supernatant in TBS/1% BSA buffer pH 7.5 with less than 0.1% sodium azide.


IHC Performance


Human Melanoma stained with anti-Vimentin antibody

Human Bladder Transitional Cell Carcinoma stained with anti-Vimentin antibody

Human Breast Ductal Cell Carcinoma stained with anti-Vimentin antibody

Human Colon Adencarcinoma stained with anti-Vimentin antibody

Human Melanoma stained with anti-Vimentin antibody

Human Bladder Transitional Cell Carcinoma stained with anti-Vimentin antibody

Human Breast Ductal Cell Carcinoma stained with anti-Vimentin antibody

Human Colon Adencarcinoma stained with anti-Vimentin antibody

Human Hepatocellular Carcinoma stained with anti-Vimentin antibody

Human Lung Squamous Cell Carcinoma stained with anti-Vimentin antibody

Human Ovary Adenocarcinoma stained with anti-Vimentin antibody

Human Prostate Adenocarcinoma stained with anti-Vimentin antibody

Human Hepatocellular Carcinoma stained with anti-Vimentin antibody

Human Lung Squamous Cell Carcinoma stained with anti-Vimentin antibody

Human Ovary Adenocarcinoma stained with anti-Vimentin antibody

Human Prostate Adenocarcinoma stained with anti-Vimentin antibody

Human Renal Cell Carcinoma stained with anti-Vimentin antibody

Human Stomach Adenocarcinoma stained with anti-Vimentin antibody

Human Tosil stained with anti-vimentin antibody

Human Renal Cell Carcinoma stained with anti-Vimentin antibody

Human Stomach Adenocarcinoma stained with anti-Vimentin antibody

Human Tosil stained with anti-vimentin antibody



Flow Cytometric Analysis

 

Flow cytometric analysis of rabbit anti-Vimentin (SP20) antibody in HeLa (green) compare to negative control of rabbit IgG (blue)
Flow cytometric analysis of rabbit anti-Vimentin (SP20) antibody in HeLa (green) compare to negative control of rabbit IgG (blue)



Western Blot Analysis

 

Western Blot analysis of rabbit anti-Vimentin (SP20) antibody
Western Blot analysis of rabbit anti-Vimentin (SP20) antibody



Product Details:


Technology

Rabbit Monoclonal

Clone Name

SP120

Application

IHC-P, WB, FACS

Species Reactivity

 

   Tested

Hu

   Predicted by homology

Bv, Ck, Fg, Hm, Ms, Rt

Isotype

Rabbit IgG

Immunogen

Recombinant human vimentin protein.

Cellular Localization

Cytoplasm

Control Tissue

Melanoma, Sarcomas

Regulatory Rating

Research Use Only (RUO)

Entrez

7431

Storage Requirement

2-8°C

Target Molecular Weight

53 kDa

Formulation

Refer to Data Sheet

Product No: 05499917001
Product No: 05298784001

Rabbit Anti-Human Vimentin Monoclonal Antibody (Clone SP20)

Instructions for Use (PDF, 92.67 KB)

Discovery XT Staining Protocol for Vimentin on Human Melanoma

Discovery-XT-Staining-Protocol (PDF, 81.07 KB)
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