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Picture of Rabbit Anti-Human Calpastatin Monoclonal Antibody (Clone SP82)

Rabbit Anti-Human Calpastatin Monoclonal Antibody (Clone SP82)

For research use only. Not for use in diagnostics.

Product No. Pack Size
06972047001
0.1 mL

Product No. Pack Size
06972071001
1.0 mL



Calpastatin is an endogenous calpain (calcium-dependent cysteine protease) inhibitor. It consists of an N-terminal domain L and four repetitive calpain-inhibition domains (domains 1-4), and it is involved in the proteolysis of amyloid precursor protein. The calpain/calpastatin system is involved in numerous membrane fusion events, such as neural vesicle exocytosis and platelet and red-cell aggregation. The protein is also thought to affect the expression levels of genes encoding structural or regulatory proteins. The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death. Calpastatin, a specific endogenous inhibitor of calpain, has a predicted molecular weight of ~77kDa and an internal repeat of four homologous domains which allow it to inhibit multiple calpain molecules simultaneously. It functions by binding to calpain when calpain binds calcium. When calpastatin is subjected to proteolytic digestion, fragments of calpastatin as small as 15 kDa still retain inhibitory activity.

Figure above:
Human Breast Carcinoma stained with anti-Calpastatin antibody

Contents

Rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.


IHC Performance


Human Breast Carcinoma stained with anti-Calpastatin antibody

Human Breast Ductal Carcinoma stained with anti-Calpastatin antibody

Human Breast stained with anti-Calpastatin antibody

Human Hepatocellular Carcinoma stained with anti-Calpastatin antibody

Human Breast Carcinoma stained with anti-Calpastatin antibody

Human Breast Ductal Carcinoma stained with anti-Calpastatin antibody

Human Breast stained with anti-Calpastatin antibody

Human Hepatocellular Carcinoma stained with anti-Calpastatin antibody

Human Bladder stained with anti-Calpastatin antibody

Human Bladder Transitional Cell Carcinoma stained with anti-Calpastatin antibody

Human Lung Adenocarcinoma stained with anti-Calpastatin antibody

Human Melanoma stained with anti-Calpastatin antibody

Human Bladder stained with anti-Calpastatin antibody

Human Bladder Transitional Cell Carcinoma stained with anti-Calpastatin antibody

Human Lung Adenocarcinoma stained with anti-Calpastatin antibody

Human Melanoma stained with anti-Calpastatin antibody

Human Prostate Adenocarcinoma stained with anti-Calpastatin antibody

Human Prostate stained with anti-Calpastatin antibody

Human Stomach Adenocarcinoma stained with anti-Calpastatin antibody

Human Prostate Adenocarcinoma stained with anti-Calpastatin antibody

Human Prostate stained with anti-Calpastatin antibody

Human Stomach Adenocarcinoma stained with anti-Calpastatin antibody



Product Details:


Technology

Rabbit Monoclonal

Clone Name

SP82

Application

IHC-P

Species Reactivity

 

   Tested

Hu

   Predicted by homology

Bv, Ck, Pg, Rb, Rt

Isotype

Rabbit IgG

Immunogen

Synthetic peptide derived from near C-terminus of human calpastatin protein.

Cellular Localization

Cytoplasm

Control Tissue

Breast Carcinoma

Regulatory Rating

Research Use Only (RUO)

Entrez

831

Storage Requirement

2-8°C

Target Molecular Weight

77 kDa

Formulation

Refer to Data Sheet

Product No: 06972047001
Product No: 06972071001

Rabbit Anti-Human Calpastatin Monoclonal Antibody (Clone SP82)

Instructions for Use (PDF, 61.41 KB)
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