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Picture of Rabbit Anti-Human B7-H3/CD276 Monoclonal Antibody (Clone SP265)

Rabbit Anti-Human B7-H3/CD276 Monoclonal Antibody (Clone SP265)

For research use only. Not for use in diagnostics.

Product No. Pack Size
07392435001
0.1 mL

Product No. Pack Size
07392451001
1.0 mL




B7-H3 (also known as CD276) is a membrane protein that participates in the regulation of T-cellmediated immune response. B7-H3 can activate both acquired and innate immunity leading to natural killer cell and CD8 T-cell dependent killing of tumor cells. High levels of B7-H3 are seen in antigen presenting cells, some tumor cells, and tumor vasculature. It is the target of anticancer immunotherapy.

Figure above:
Human ovarian adenocarcinoma stained with anti-B7-H3 antibody

Contents

Rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.


IHC Performance


Human ovarian adenocarcinoma stained with anti-B7-H3 antibody

Human lung squamous cell carcinoma stained with anti-B7-H3 antibody

Human renal cell carcinoma stained with anti-B7-H3 antibody

Human liver hepatoma stained with anti-B7-H3 antibody

Human ovarian adenocarcinoma stained with anti-B7-H3 antibody.

Human lung squamous cell carcinoma stained with anti-B7-H3 antibody.

Human renal cell carcinoma stained with anti-B7-H3 antibody

Human liver hepatoma stained with anti-B7-H3 antibody

Human bladder transitional cell carcinoma stained with anti-B7-H3 antibody

Human cervical squamous cell carcinoma stained with anti-B7-H3 antibody

Human skin squamous cell carcinoma stained with anti-B7-H3 antibody

Human skin melanoma stained with anti-B7-H3 antibody

Human bladder transitional cell carcinoma stained with anti-B7-H3 antibody

Human cervical squamous cell carcinoma stained with anti-B7-H3 antibody

Human skin squamous cell carcinoma stained with anti-B7-H3 antibody

Human skin melanoma stained with anti-B7-H3 antibody

Human Hodgkin's lymphoma stained with anti-B7-H3 antibody

Human prostate adenocarcinoma stained with anti-B7-H3 antibody

Human Hodgkin's lymphoma stained with anti-B7-H3 antibody

Human prostate adenocarcinoma stained with anti-B7-H3 antibody



Flow Cytometric Analysis


Flow cytometric analysis of rabbit anti-B7-H3/CD276 (SP265) antibody in PC-3 cells (green) compare to negative control of rabbit IgG (blue)
Flow cytometric analysis of rabbit anti-B7-H3/CD276 (SP265) antibody in PC-3 cells (green) compare to negative control of rabbit IgG (blue)


Western Blot Analysis


Western Blot analysis of Hs700T cell lysate with B7-H3/CD276 antibody
Western Blot analysis of Hs700T cell lysate with B7-H3/CD276 antibody


Product Details:


Technology

Rabbit Monoclonal

Clone Name

SP265

Application

IHC-P, WB, FACS

Species Reactivity

 

   Tested

Hu

   Predicted by homology

Ms, Rt

Isotype

Rabbit IgG

Immunogen

Synthetic peptide derived from the C-terminus of human B7-H3/CD276 protein.

Cellular Localization

Membrane

Control Tissue

Ovarian Adenocarcinoma

Regulatory Rating

Research Use Only (RUO)

Entrez

80381

Storage Requirement

2-8°C

Target Molecular Weight

110 kDa

Formulation

Refer to Data Sheet

Product No: 07392435001
Product No: 07392451001

Rabbit Anti-Human B7-H3/CD276 Monoclonal Antibody (Clone SP265)

Instructions for Use (PDF, 74.22 KB)
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