LightCycler® TaqMan® Master
Ready-to-use hot start reaction mix for PCR using the LightCycler® Carousel-Based System Instruments with hydrolysis probes.
For life science research only. Not for use in diagnostic procedures.
Ready-to-use Hot Start Reaction Mix for PCR, using TaqMan® probes with the LightCycler® Carousel-Based System.
- Save time with a convenient, ready-to-use 5x concentrated hot start master mix.
- Get reliable results with a master mix specifically designed for use with Universal ProbeLibrary probes on the LightCycler® Carousel-Based System.
- Eliminate time-consuming MgCl2 titration.
- Achieve consistent, high quality performance with the LightCycler® Carousel-Based System Instruments.
- LightCycler® FastStart Enzyme
- LightCycler® FastStart TaqMan® Reaction Mix
- Water, PCR Grade
The LightCycler® TaqMan® Master is designed for quantitative PCR or two-step RT-PCR using the LightCycler® Carousel-Based System Instruments for hydrolysis probe assays (e.g., Universal ProbeLibrary assays). Use the LightCycler® TaqMan® Master with heat-labile Uracil-DNA Glycosylase for carryover prevention during PCR.
Note: The hydrolysis probe assay is based on probe cleavage during PCR; therefore, hydrolysis probes cannot be used to perform melting curve analysis.
LightCycler®TaqMan® Master is a ready-to-use reaction mix designed for the hydrolysis probe detection format in LightCycler® Capillaries using the LightCycler® Carousel-Based System. It contains FastStart Taq DNA Polymerase for hot start PCR, which significantly improves the specificity and sensitivity of PCR by minimizing the formation of nonspecific amplification products.
Hydrolysis probe assays, also called TaqMan®assays, use a single probe containing two labels: a fluorescent reporter dye and a fluorescent quencher. While the probe is intact, the quencher is close to the reporter dye and suppresses the reporter fluorescence via fluorescence resonance energy transfer (FRET). When the probe is hybridized to the target sequence, the 5'-nuclease activity of the polymerase cleaves the hydrolysis probe, separating the reporter and quencher. With an increasing amount of target sequence during PCR, more probe is cleaved, and the fluorescence signal of the unquenched reporter dye increases.
Function test: The LightCycler® TaqMan® Master is function tested using the LightCycler® Carousel-Based System Instruments.