LightCycler® RNA Amplification Kit SYBR Green I
Kit for one-step RT-PCR using the LightCycler® Carousel-Based System.
For life science research only. Not for use in diagnostic procedures.
The kit provides reagents, including enzyme mix, reaction mix with SYBR Green I dye, MgCl2, and PCR-grade water for amplification and detection of RNA using the LightCycler® Carousel-Based System Instruments.
The LightCycler® RNA Amplification Kit SYBR Green I provides convenience, high performance, reproducibility, and minimized contamination risk. Addition of MgCl2 (provided in the kit) may be necessary to optimize performance.
- LightCycler® RT-PCR Enzyme Mix
- LightCycler® RT-PCR Reaction Mix SYBR Green I
- MgCl2 Stock Solution, 25 mM
- Water, PCR-grade
- Resolution Solution
The LightCycler® RNA Amplification Kit SYBR Green I is specifically adapted for one-step RT-PCR in LightCycler® Capillaries using SYBR Green I dye as detection format.
SYBR Green I is a DNA double-strand-specific dye. During each phase of DNA synthesis, the dye binds to the amplified PCR products; the amplicon can be detected by its fluorescence. Combining amplification with melting-curve analysis can enhance specificity and sensitivity of amplification reactions.
The LightCycler® RNA Amplification Kit SYBR Green I is function tested using the LightCycler® Control Kit RNA, according to the kit protocols.