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LightCycler® EvoScript RNA Probes Master

The LightCycler® EvoScript RNA Probes Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.

For general laboratory use.

Product No. Pack Size List Price Your Price Quantity
07800029001
200 x 20 µL reactions $ 523.00

Product No. Pack Size List Price Your Price Quantity
07800096001
1000 x 20 µL reactions $ 2,405.00

The LightCycler® EvoScript RNA Probes Master (1-vial composition) is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays.
 

  • Ease of use, convenience, speed, reduced error
  • Result consistency, stability and tolerance of targets of varying secondary structure
  • Ability to detect low copy or rare transcripts for gene expression applications
  • Superior quantification capability for gene expression applications
  • Easier assay optimization
  • Contamination control capability
  • Generate consistent and robust results, no matter what sample type you are starting from or what target you are detecting
  • Rely on a higher sensitivity when detecting low copy numbers, with the input of larger sample volumes into the reaction
  • Obtain fast and specific results

Contents


  1. Master Mix, 5× conc. Contains enzymes for reverse transcription and PCR, RT-qPCR Reaction Buffer, dATP, dCTP, dGTP, and dUTP, Mg(OAc)2, and proprietary additive

    07 800 029 001   2 vials, 440 μL each
    07 800 096 001 10 vials, 440 μL each

  2. Water, PCR grade To adjust the final reaction volume.

    07 800 029 001   3 vials, 1 mL each
    07 800 096 001   2 vials, 7.25 mL each

The LightCycler® EvoScript RNA Probes Master is designed for sensitive, high-specificity, high-precision one-step RT-qPCR especially for gene expression assays. The 1-vial composition is ideally suited for easy reaction assembly, requiring only the addition of primers, probe and target RNA. The proprietary reaction buffer allows a convenient hot start RT-qPCR.

In addition, the enzymes for reverse transcription and for PCR feature hot start for premium specificity1).
The master mix is optimized for hydrolysis probes, including Universal ProbeLibrary (UPL) probes and does not require optimization with Mg(OAc)2.

There are protocols for both the LightCycler® 480 instrument and the LightCycler® 96 instrument.

1) The reverse transcription activity will be suppressed at temperatures significantly below 60°C.

Product No: 07800029001
Product No: 07800096001
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