LightCycler® DNA Master HybProbe
Easy-to-use fast high-performance qPCR kit for HybProbe signal transmission with all LightCycler® Carousel-Based Instruments.
For general laboratory use.
Easy-to-use reaction mix for PCR, using HybProbe probes with the LightCycler® Carousel-Based System.
LightCycler® DNA Master HybProbe provides robust performance and convenience for standard PCR applications.
- LightCycler® DNA Master HybProbes
- MgCl2 Stock Solution, (25 mM)
- Water, PCR-grade
LightCycler® DNA Master HybProbe is an easy-to-use reaction mix for sensitive PCR applications in LightCycler® capillaries, using HybProbe probes as detection format. The kit can be used to perform quantitative PCR, as well as SNP and mutation detection. The addition of MgCl2 (provided in the kit) for specific applications may be necessary.
The unique LightCycler® HybProbe format is based on the principle of fluorescence resonance energy transfer (FRET). Two sequence-specific oligonucleotide probes, labeled with different dyes (donor and acceptor), are added to the reaction mix in addition to PCR primers. HybProbe probes hybridize to the target sequences on the amplified DNA fragment during the annealing phase in a head-to-tail arrangement, thereby bringing the two dyes into close proximity. The donor dye (fluorescein) is excited by the blue light LED source and the energy emitted by the donor dye excites the acceptor dye attached to the second HybProbe probe, which then emits fluorescent light at a different wavelength. The amount of fluorescence is directly proportional to the amount of target DNA generated during the PCR process.
Note: All LightCycler® Master Mixes have been specifically developed to function in LightCycler® Capillaries and provide optimal performance and sensitivity.
The LightCycler® DNA Master HybProbe is function tested using the LightCycler® Control Kit DNA, according to the kit protocols.