LightCycler® Color Compensation Set
Ready-to-use solutions for the generation of color-compensation files on the LightCycler® Carousel-Based System Instruments.
For general laboratory use.
Ready-to-use solutions for the generation of color compensation files for the LightCycler® Carousel-Based System.
- Blank, standard buffer excluding fluorophores; to determine the standard buffer fluorescence
- Fluorescein Calibrator, standard oligonucleotide, fluorescein-labeled at the 3' end [0.3 µM]; to determine the crosstalk from fluorescein (channel 1 fluorophore) into channels 2 and 3
- LightCycler® Red 640 Calibrator, standard oligonucleotide, LightCycler® Red 640-labeled at the 3' end [1 µM]; to determine the crosstalk from LightCycler® Red 640 (channel 2 fluorophore) into channels 1 and 3
- Cy5.5 Calibrator, standard oligonucleotide, Cy5.5-labeled at the 3' end [1 µM]; to determine the crosstalk from Cy5.5 (channel 3 fluorophore) into channels 1 and 2
The color-compensation files, generated with the LightCycler® Color Compensation Set, are a prerequisite to performing dual-color experiments with both LightCycler® Red 640-labeled and Cy5.5-labeled HybProbe probes in a single capillary.
Although the optical filters of each detection channel of the LightCycler® Instruments are optimized for the different emission maxima of Fluorescein, LightCycler® Red 640, and Cy5.5 dye, some residual crosstalk between the channels is observed. This crosstalk can be corrected using a color-compensation file.
The LightCycler® Color Compensation Set provides ready-to-use solutions for the generation of color-compensation files to compensate for crosstalk. The crosstalk is observed between single channels when using differently labeled HybProbe probes in multiplex dual-color experiments (i.e., in one capillary). The generated color-compensation file can be used in all subsequent dual-color experiments on that specific LightCycler® Carousel-Based System Instrument to compensate for the raw fluorescence data, either online during the run or for data analysis after the run.