Log In

Please provide Log in ID
Please provide Password
Forgot password  Forgot user ID?

No Account Yet?

This website uses cookies to provide you with a more responsive and personalized service. In order to proceed to login, however, you must formally accept our cookie policy. Please read our cookie & privacy policies for more information.

High Pure PCR Product Purification Kit

For life science research only. Not for use in diagnostic procedures.

Product No. Pack Size List Price Your Price Quantity
11732668001
50 purifications $ 89.00

Product No. Pack Size List Price Your Price Quantity
11732676001
250 purifications $ 400.00

The High Pure PCR Product Purification Kit efficiently and conveniently isolates PCR products from amplification reactions and purifies nucleic acids from other modification reactions. It is also recommended for the purification of cDNA.

  • Quickly purifies multiple PCR products
    in <10 minutes, and purify DNA from agarose gel slices in <20 minutes.
  • Efficiently recover DNA fragments
    >100 bp in length, and concentrate dilute nucleic acid solutions.
  • Minimize DNA loss
    with a kit that removes contaminants without precipitation or other handling steps that degrade DNA.
  • Eliminate the use of hazardous organic compounds
    such as cesium chloride, phenol, chloroform, and ethidium bromide (see Figure 1).

 

Contents

 

  1. Binding Buffer
  2. Wash Buffer
  3. Elution Buffer
  4. High Pure Spin Filter Tubes (containing glass fiber fleece)
  5. Collection Tubes

 

The High Pure PCR Product Purification Kit is designed for the preparation of concentrated, purified DNA, and can be used directly for most molecular biology applications:

  • Labeling
  • Sequencing
  • Cloning
  • Restriction enzyme digests
  • Alkaline phosphatase treatment
  • Kinase reactions

The kit eliminates primers, mineral oil, salts, unincorporated nucleotides, and thermostable DNA polymerases, which may inhibit subsequent enzymatic reactions. It can also be applied to concentrate dilute nucleic-acid solutions. Use one kit for a variety of applications.

The fast and simple High Pure protocols use a tabletop centrifuge to bind, wash, and elute the reaction product down to 10 µl (micro format) in as little as 10 minutes. The procedure conveniently eliminates a concentration step, and is ideal for downstream applications such as labeling, sequencing, cloning, ligation, or amplification using PCR.


 


Choose the right product for DNA fragment purification.


 

High Pure PCR Product Purification Kit

High Pure PCR Cleanup Micro Kit

Purification from agarose gel slices

X

 

Purification from reaction mixes

X

X

Concentration in 50 µl

X

 

Concentration in 10 µl

 

X

Depletion of primer-dimers <100 bp

 

X

DNA fragments up to 50 kb

X

 

DNA fragments up to 5 kb

 

X

 

 

Figure 1: Comparison of PCR products purified with the High Pure PCR Product Purification Kit and those purified by phenol/chloroform extraction. Four PCR products of different length were generated with the Expand Long Template PCR System. These were purified by either method, then analyzed electrophoretically. From left to right, the four products shown on the gel are: a 2.9 kb fragment from the p53 gene; 4.8 kb, 6.3 kb, and 9.3 kb fragments from the tissue plasminogen activator (tPA) gene.

Lanes 1: DNA before purification
Lanes 2: DNA after purification by phenol/chloroform extraction
Lanes 3: DNA after purification with the High Pure PCR Product Purification Kit

Result: The High Pure PCR Product Purification Kit removed primers and primer-dimers, while recovering 90% of the long PCR products (calculated by photospectroscopy).

Nucleic acids bind to the surface of the glass fiber fleece in the presence of a chaotropic salt (guanidine HCl). This allows the High Pure filter tube to specifically immobilize nucleic acids (both DNA and RNA) while they are freed of contaminants.

Capacity: The High Pure SpinFilter Tubes hold up to 700 μl sample volume.
Volume: 100 μl
Typical samples include:

  • Products from amplification or cDNA synthesis reactions
  • Enzymatically treated DNA
  • DNA from agarose slices
  • Dilute nucleic acid solutions
  • RNA from transcription reactions

 

Principle

The sample is mixed with a chaotropic salt and applied to the glass fiber fleece in a High Pure Spin Filter Tube. Under the buffer conditions used in the procedure, all nucleic acids (NA) in the sample bind to the glass fleece in the High Pure tube, while contaminating substances (salts, proteins, nucleotides, mineral oil and other contaminants) do not. Brief wash-and-spin steps readily remove these contaminants. Once purified, the NA can be easily eluted in a small volume of low salt buffer. 

Quality

More than 70% recovery is obtained when 10 μg DNA Molecular Weight Marker VIII mixed with 16 μg bovine serum albumin are applied to the High Pure Filter Tubes. Gel electrophoresis of the DNA eluate confirms the complete removal of protein and DNA fragments smaller than 100 bp.

Product No: 11732668001
Product No: 11732676001

Cloning and Gene Synthesis

High Pure Technology and Silica Adsorption Kits

High Pure PCR Product Purification Kit: Changed Protocol for Purification of Large DNA Fragments (4.5 kb to >30 kb)

Article (PDF, 260.57 KB)

Nucleic Acid Isolation and Purification Product Selection Guide

Article (PDF, 773.44 KB)
true