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FastStart Universal Probe Master (Rox)

Universal ready-to-use hot start reaction mix for qPCR and RT-qPCR on all real-time PCR systems requiring normalization with ROX.

For life science research only. Not for use in diagnostic procedures.

Product No. Pack Size List Price Your Price Quantity
04913949001
250 x 20 µL reactions $ 161.00

Product No. Pack Size List Price Your Price Quantity
04913957001
1250 x 20 µL reactions $ 751.00

Product No. Pack Size List Price Your Price Quantity
04914058001
5000 x 20 µL reactions $ 2,700.00

FastStart Universal Probe Master (Rox) includes a novel reference dye that enables its use on all real-time PCR instruments requiring normalization with ROX, without modification or adjustments to the specific instrument or protocol. This ready-to-use, 2x concentrated master mix contains all reagents (except primers, probe, and template) needed for running quantitative, real-time DNA-detection assays, including qPCR and two-step qRT-PCR, in the hydrolysis probe detection format. FastStart Universal Probe Master (Rox) generates excellent results on instruments such as the Applied Biosystems 7900 HT Fast Real-Time PCR System (see Figure 1) or the Applied Biosystems 7500 Real-Time PCR System. This product is not intended for use with the LightCycler® Instruments.

  • Increase qPCR sensitivity and specificity.
    Produce lower cycle threshold (Ct) values.
  • Use the master mix with any probe-based assay .
    Achieve sensitive, specific results in assays with the Universal ProbeLibrary Probes or any other hydrolysis probe.
  • Amplify and detect a broad range of DNA or cDNA targets.
    Amplify fragments up to 500 bp long, including those that are GC- or AT-rich.
  • Visualize amplification products on agarose gels.
  • Use robotic pipetting stations to set up qPCR reactions.
    Use a master mix that is stable at room temperature during extended reaction setup times.
  • Prevent false positives resulting from carryover contamination.
    Use this dUTP-containing mix with Uracil-DNA Glycosylase to eliminate contaminating DNA carried over from previous PCR reactions. 

Contents

FastStart Universal Probe Master (Rox), 2x concentrated master mix that contains FastStart Taq DNA Polymerase, Reaction Buffer, Nucleotides (dATP, dCTP, dGTP, dUTP), and a reference dye.

FastStart Universal Probe Master (Rox) can be used for the amplification and detection of any DNA or cDNA target, including those that are GC- or AT-rich. Combine this master mix with Transcriptor First Strand cDNA Synthesis Kit (Roche) to achieve excellent results in two-step qRT-PCR.



Figure 1: Performance of FastStart Universal Probe Master on an ABI 7900 Instrument. The data show a comparison of Roche FastStart Universal Probe Master (a) and a master mix from another supplier (b). Reactions use a hydrolysis probe specific for the globin gene and a titration of human genomic DNA at concentrations of 50, 5, 0.5, and 0 ng/well. FastStart Universal Probe Master (Rox) data demonstrates earlier Ct -values and consistent, sensitive results.

Hot start protocols have been shown to significantly improve the specificity, sensitivity, and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of FastStart Taq DNA Polymerase make the modified enzyme inactive at room temperature. Therefore, there is no elongation during the period when primers can nonspecifically bind. FastStart Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e., a preincubation step at +95°C).
 

Quality

Function test: Each lot is tested for performance in qPCR using three templates: a GC–rich template, an AT-rich template, and a long template (approximately 440 bp).

Product No: 04913949001
Product No: 04913957001
Product No: 04914058001

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