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Picture of FastStart Essential DNA Green Master

FastStart Essential DNA Green Master

Ready-to-use hot start reaction mix for SYBR Green I-based real-time PCR using the LightCycler® 96 Instrument and the LightCycler® Nano Instrument.

For life science research only. Not for use in diagnostic procedures.

Product No. Pack Size List Price Your Price Quantity
06402712001
500 x 20 µL reactions $ 360.00

Product No. Pack Size List Price Your Price Quantity
06924204001
5000 x 20 µL reactions $ 3,121.00

The FastStart Essential DNA Green Master is a one-component hot start reaction mix for qualitative and quantitative PCR. It contains FastStart Taq DNA Polymerase and double-stranded DNA specific SYBR Green I dye for product detection and characterization. The mix is provided as an easy-to-use master reagent. Reaction setup only requires the addition of template DNA and primers. The mix can be used with different types of DNA (e.g., genomic, cDNA).

 

  • Improve specificity, sensitivity, and PCR yield with hot start protocols.
  • Achieve consistent, high-quality performance with the LightCycler® 96 and Nano Instruments using Multiwell Plates, 96  or 8-Tube Strips.
  • Save time with a convenient, ready-to-use 2x concentrated hot start master mix.

 

Contents

  1. FastStart Essential DNA Green Master, 2x concentrated 
  2. Water, PCR Grade

 

The FastStart Essential DNA Green Master provides reagents, including SYBR Green I dye, for amplification and detection of DNA using the LightCycler® 96 Instrument and the LightCycler® Nano Instrument. The kit is ideally suited for hot start PCR assays for gene detection and quantification.

Note: The FastStart Essential DNA Green Master can be used in conjunction with heat-labile LightCycler® Uracil DNA Glycosylase for carryover prevention during PCR. It is ideal for two-step RT-PCR applications, for example, when used downstream of cDNA synthesis using Transcriptor Reverse Transcriptase.

SYBR Green I is a double-stranded DNA specific dye. During each phase of DNA synthesis, SYBR Green I dye which is included in the reaction mix, binds to the amplified PCR products. Resulting amplicons can then be detected by their fluorescence.
Hot start protocols used with the FastStart Essential DNA Green Master have been shown to significantly improve specificity, sensitivity, and PCR yield. Heat-labile blocking groups on some of the amino acid residues of FastStart Taq DNA Polymerase make the modified enzyme inactive at room temperature. This means that there is no elongation during the period when primers can nonspecifically bind. FastStart Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e., a pre-incubation step at +95°C for 10 minutes).

Note: The FastStart Essential DNA Green Master can be used in conjunction with heat-labile LightCycler® Uracil DNA Glycosylase  for carryover prevention during PCR. It is ideal for two-step RT-PCR applications, for example, when used downstream of cDNA synthesis using Transcriptor Reverse Transcriptase.

Product No: 06402712001
Product No: 06924204001