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DNA Isolation Kit for Cells and Tissues

For life science research only. Not for use in diagnostic procedures.

Product No. Pack Size List Price Your Price Quantity
11814770001
10 isolations $ 294.00

The DNA Isolation Kit for Cells and Tissues provides medium- and large-scale preparation of purified genomic DNA ranging in size from 50 to 150 kb. Remove contaminating RNA and proteins from a wide variety of biological specimens (mammalian tissue, cultured cells, yeast, gram-negative bacteria or mouse tail).

  • Obtain increased yields of DNA in less than 2.5 hours.
    Benefit from a simple, straightforward procedure (compared with column based methods).
  • Increase safety and convenience.
    Eliminate the use of chaotropic salts, anion-exchange columns, and hazardous organic solvents.
  • Reduce purification time.
    All required reagents are included in the kit.

Contents

  1. Cellular Lysis Buffer
  2. Proteinase K Solution
  3. RNase Solution
  4. Protein Precipitation Solution

Use DNA Isolation Kit for Cells and Tissues to isolate DNA from a wide variety of starting materials. The isolated DNA is suitable for many molecular biology applications:

  • Genomic Southern blotting
  • Sequencing
  • Restriction digestion
  • PCR/long PCR (see Figure 1)
  • Cloning

 



Figure 1: Amplification of both short and long DNA fragments from genomic DNA prepared with the DNA Isolation Kit for Cells and Tissues.  Genomic DNA was isolated from a variety of sources and then amplified with either Taq DNA Polymerase, the Expand High Fidelity PCR System, or the Expand Long Template PCR System.
Lanes 2, 3: Human DMD fragment (268 bp) and mouse c-myc fragment (580 bp), amplified with Taq DNA Polymerase
Lanes 4, 5, 7, 8: Human c-myc fragment (1.2 kb), mouse β2-microglobulin fragment (3.6 kb), bovine lysozyme gene fragment (6.9 kb), and human tPA gene fragment (9.3 kb), all amplified with the Expand High Fidelity PCR System
Lanes 6, 9, 10: Mouse α-2 collagen gene fragments (5.6 kb and 10.4 kb) and human β-globin fragment (23 kb), amplified with the Expand Long Template PCR System
Lanes 1, 11 : DNA Molecular Weight Markers VI and II

Typical DNA Recovery

Starting Material and Quantity

Yield/Recovery

Time Required

Number of Reactions

Tissue, 100 mg - 1g

Depending on tissue type

<2.5 hours

10/400 mg

Cultured cells, 1 x 107 -5 x 107

700-3000 µg/5 x 107 cells

   

Mouse tail, 50 - 400 mg

800 µg/400 mg mouse tail

   

Yeast, up to 3 x 105

300 µg/3 x 1010 yeast cells

   

Gram negative bacteria, up to 1 x 1011

1 500 - 2750 µg/1011 bacteria

   


 

Purity of isolated DNA: Average A 260 / A 280 ratio: 1.7 - 1.9

Isolation of High Molecular Weight DNA
The kit simplifies the isolation of 50 to 150 kb genomic DNA.


Principle

Sample material is homogenized in Cellular Lysis Buffer in the presence of a strong anionic detergent and proteinase K. RNA is eliminated by RNase treatment and proteins are removed by selective precipitation and centrifugation. The purified DNA is finally recovered from solution by isopropanol precipitation.
 

Quality

Each lot of the DNA Isolation Kit for Cells and Tissues is tested for the absence of DNase contamination. Function tests to purify DNA from bovine liver, followed by specific amplification with the Expand High Fidelity PCR System were performed.

Product No: 11814770001

Nucleic Acid Isolation and Purification Product Selection Guide

Article (PDF, 773.44 KB)
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