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Agarose Gel DNA Extraction Kit

For the elution of DNA fragments from agarose gels.

For life science research only. Not for use in diagnostic procedures.

Product No. Pack Size List Price Your Price Quantity
11696505001
100 reactions $ 142.00

The Agarose Gel DNA Extraction Kit provides convenient isolation of high-quality DNA in large amounts.

  • Quick and simple.
    Extract high yields of DNA in only 45 minutes, with few hands-on steps.
  • Combine with different agaroses and buffer systems.
    Low melting point agarose is not required.€
  • Purify efficiently.
    Highly specific binding of DNA allows easy removal of impurities.
  • Isolate large DNA fragments without shearing.
    Silica particles are uniform in size and have smooth surfaces, ensuring recovery of intact DNA fragments up to 100 kb.
  • Isolate oligonucleotides ≥20 bp.
    Narrow size distribution of the silica particles and absence of fines ensure high binding capacity of the matrix.
  • Avoid enzymatic inhibition.
    No fine particles are observed in the suspension. The recovered product will not inhibit subsequent enzymatic reactions. 

Contents

  1. Silica Matrix, for 100 standard reactions
  2. Solubilization Buffer
  3. Nucleic Acid Binding Buffer
  4. Wash Buffer 

The Agarose DNA Extraction Kit efficiently isolates both small and large DNA fragments from standard or low melting point agarose (see Figure 1). Recovered DNA fragments are suitable for:

  • Ligation and transformation
  • Enzymatic restriction
  • Random primed or nick translation labeling methods
  • Sequencing 
  • PCR/long PCR
  • Cloning
  • Concentrate dilute nucleic acid solutions

 



Figure 1: Recovery of DNA fragments from agarose gels with the Agarose DNA Extraction Kit. DNA fragments (0.5 to 23 kb) from Molecular Weight Marker II (Roche) were separated on a 1% agarose gel, then extracted from the gel with the Agarose Gel DNA Extraction Kit. Isolated fragments were displayed on a new agarose gel.

Lanes 1, 9: MWM II
Lane 2: 23 kb fragment
Lane 3: 9.5 kb fragment
Lane 4: 6.5 kb fragment
Lane 5: 4.3 kb fragment
Lane 6: 2.3 kb fragment
Lane 7: 2.0 kb fragment
Lane 8: 0.5 kb fragment

Result: The Agarose Gel DNA Extraction Kit isolated both large and small DNA fragments with good yields.

By combining agarose gel electrophoresis and extraction, you can easily concentrate dilute, aqueous DNA solutions. After processing with the kit, the DNA can be recovered in a volume of 20 to 50 μL. Isolated DNA fragments are free of inhibitors that could affect common downstream procedures. For example, recovered DNA can be efficiently ligated into cloning vectors or labeled to high specific activity. Restriction digests proceed without inhibition.

Typical DNA Recovery

Starting Material and Quantity

Yield/Recovery*

Time Required

Number of Reactions

100 mg gel slice
(1 standard reaction)

20 bp - 55%

60 minutes

Up to 100/100 mg agarose

 

40 bp - 68%

   
 

120 bp - 76%

   
 

200 bp - 80%

   
 

8-9 kb - 75%

   
 

>10 kb - 60%

   

*Size range of recovered DNA: 20 bp to 100 kb
 

Principle

A solubilization buffer containing a chaotropic salt (sodium perchlorate) dissolves an agarose gel slice that contains a DNA fragment. In the presence of the chaotropic salt, the DNA fragment binds selectively to silica matrix. The DNA remains bound while a series of rapid wash-and-spin steps remove contaminating small molecules. Finally, low-salt elution removes the DNA from the silica matrix. The process does not require DNA precipitation, organic solvent extractions, or extensive handling of the DNA.
 


Quality

DNA Molecular Weight Marker II is separated in a 0.8% agarose gel and the 546-, 2,322-, and 6,557 bp fragments are isolated with the kit components. The expected amount of DNA is recovered for each fragment. Depending on parameters such as fragment length, preparation, and purity of the applied DNA, up to 80% recovery is achieved. DNA fragments are digested with restriction enzymes. No inhibition of DNA digestion is observed.

Product No: 11696505001

High Pure Technology and Silica Adsorption Kits

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